Master 2 Internship

Research topic

Influence of microbial chemical cues on the settlement behaviour of larval and juvenile fishes around coastal and artificial habitats

Laboratory

UMR 7232 CNRS-SU, Biologie Intégrative des Organismes Marins (BIOM), EcoEvoDevo Team

UMR 8176 CNRS-SU-UPVD, Laboratoire de Biodiversité et Biotechnologies des Microorganismes (LBBM)

Address

Observatoire Océanologie de Banyuls-sur-Mer, 1 avenue Pierre Fabre, Banyuls-sur-Mer, France

Dates

January to July 2027

Stipend

Yes: ~ 600€ / month (French students)

Summary

Marine environments are undergoing rapid transformation as a result of increasing human activities and the development of offshore infrastructures. Understanding the mechanisms that govern the colonisation of new habitats by marine organisms has therefore become a major challenge in marine ecology.

In most teleost fishes, larvae initially disperse offshore before returning towards coastal environments to settle in habitats suitable for their growth as juveniles. The increasing deployment of artificial structures at sea, particularly in the context of marine renewable energy development, is creating new habitats that can be colonised by recruiting larval fishes.

However, the cues involved in the settlement of larval fishes within these habitats remain poorly understood, despite their potentially important role in shaping these novel marine ecosystems. Among the environmental cues that may guide fishes during settlement, chemical cues are particularly relevant because they can potentially be detected over relatively long distances.

Submerged structures are rapidly colonised by biofouling communities, comprising the macro- and microorganisms growing on immerged surfaces. These biological communities release a wide range of chemical compounds into the surrounding environment. This internship will investigate the hypothesis that some compounds produced by biofouling communities – particularly molecules involved in microbial chemical communication – may act as cues influencing the settlement of larval fishes around both coastal and artificial habitats.

The main objective of the internship will be to investigate the effects of compounds involved in quorum sensing, a chemical communication mechanism among microorganisms, on larval fish behaviour and to contribute to identifying their mechanisms of action.

The first part of the internship will focus on behavioural ecology. The intern will participate in the sampling and husbandry of larval and juvenile common two-banded seabream (Diplodus vulgaris). Behavioural experiments will then be conducted using double choice flume tanks, in which fish will be exposed to different quorum-sensing-related compounds to assess their behavioural responses. Videos recorded during these experiments will subsequently be analysed using automated trajectory-tracking approaches involving pose estimation using deep learning models.

In a second part, a laboratory bioassay will be conducted in which D. vulgaris will be exposed to the compounds previously tested in the behavioural experiments. The effects of these compounds on the expression of several genes encoding candidate receptors will then be investigated to better understand their mechanisms of action in fish. Gene expression will be quantified using digital PCR.

Through this internship, the student will gain experience in behavioural ecology, chemical ecology and molecular biology, as well as in data processing, statistical analyses and the communication of scientific results.

Related publications

  • Havel L.N., Fuiman L.A. 2016. Settlement-Size Larval Red Drum (Sciaenops ocellatus) Respond to Estuarine Chemical Cues. Estuaries and Coasts.

  • Jutfelt F., Sundin J., Raby G.D., et al. 2017. Two-current choice flumes for testing avoidance and preference in aquatic animals. Methods in Ecology and Evolution.

  • Tait K., Havenhand J. 2013. Investigating a possible role for the bacterial signal molecules N-acylhomoserine lactones in Balanus improvisus cyprid settlement. Molecular Ecology.

How to apply?

Contact supervisor(s) with a CV, a Cover Letter, and references if relevant. Position opened until filled.